Biochemical characterization of baculovirus-expressed rap1A/Krev-1 and its regulation by GTPase-activating proteins.

Normal human rap1A and 35A rap1A (which encodes a protein with a Thr-35----Ala mutation) were cloned into a baculovirus transfer vector and expressed in Sf9 insect cells. The resulting proteins were purified, and their nucleotide binding, GTPase activities, and responsiveness to GTPase-activating pr...

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Bibliografiske detaljer
Main Authors: Quilliam, L A, Der, C J, Clark, R, O'Rourke, E C, Zhang, K, McCormick, F, Bokoch, G M
Format: Artigo
Sprog:Inglês
Udgivet: 1990
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC360652/
https://ncbi.nlm.nih.gov/pubmed/2160589
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