Biochemical characterization of baculovirus-expressed rap1A/Krev-1 and its regulation by GTPase-activating proteins.

Normal human rap1A and 35A rap1A (which encodes a protein with a Thr-35----Ala mutation) were cloned into a baculovirus transfer vector and expressed in Sf9 insect cells. The resulting proteins were purified, and their nucleotide binding, GTPase activities, and responsiveness to GTPase-activating pr...

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Vydáno v:	Mol Cell Biol
Hlavní autoři:	Quilliam, L A, Der, C J, Clark, R, O'Rourke, E C, Zhang, K, McCormick, F, Bokoch, G M
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	Taylor & Francis 1990
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360652/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2160589/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.6.2901-2908.1990
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