Biochemical characterization of baculovirus-expressed rap1A/Krev-1 and its regulation by GTPase-activating proteins.

Normal human rap1A and 35A rap1A (which encodes a protein with a Thr-35----Ala mutation) were cloned into a baculovirus transfer vector and expressed in Sf9 insect cells. The resulting proteins were purified, and their nucleotide binding, GTPase activities, and responsiveness to GTPase-activating pr...

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Publicado en:	Mol Cell Biol
Autores principales:	Quilliam, L A, Der, C J, Clark, R, O'Rourke, E C, Zhang, K, McCormick, F, Bokoch, G M
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	Taylor & Francis 1990
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360652/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2160589/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.6.2901-2908.1990
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