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Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels

BACKGROUND: In Escherichia coli many heterologous proteins are produced in the periplasm. To direct these proteins to the periplasm, they are equipped with an N-terminal signal sequence so that they can traverse the cytoplasmic membrane via the protein-conducting Sec-translocon. For poorly understoo...

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Autors principals: Schlegel, Susan, Rujas, Edurne, Ytterberg, Anders Jimmy, Zubarev, Roman A, Luirink, Joen, de Gier, Jan-Willem
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605120/
https://ncbi.nlm.nih.gov/pubmed/23497240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-12-24
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