Family-Specific Degenerate Primer Design: A Tool to Design Consensus Degenerated Oligonucleotides

Designing degenerate PCR primers for templates of unknown nucleotide sequence may be a very difficult task. In this paper, we present a new method to design degenerate primers, implemented in family-specific degenerate primer design (FAS-DPD) computer software, for which the starting point is a mult...

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Detalhes bibliográficos
Main Authors: Iserte, Javier Alonso, Stephan, Betina Ines, Goñi, Sandra Elizabeth, Borio, Cristina Silvia, Ghiringhelli, Pablo Daniel, Lozano, Mario Enrique
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2013
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3600133/
https://ncbi.nlm.nih.gov/pubmed/23533783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2013/383646
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