Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences.

We describe a new primer design strategy for PCR amplification of unknown targets that are related to multiply-aligned protein sequences. Each primer consists of a short 3' degenerate core region and a longer 5' consensus clamp region. Only 3-4 highly conserved amino acid residues are necessary for...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Rose, T M, Schultz, E R, Henikoff, J G, Pietrokovski, S, McCallum, C M, Henikoff, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1998
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC147464/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9512532/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/26.7.1628
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