Consensus-degenerate hybrid oligonucleotide primers for amplification of distantly related sequences.

We describe a new primer design strategy for PCR amplification of unknown targets that are related to multiply-aligned protein sequences. Each primer consists of a short 3' degenerate core region and a longer 5' consensus clamp region. Only 3-4 highly conserved amino acid residues are nece...

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Main Authors: Rose, T M, Schultz, E R, Henikoff, J G, Pietrokovski, S, McCallum, C M, Henikoff, S
Format: Artigo
Jezik:Inglês
Izdano: 1998
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC147464/
https://ncbi.nlm.nih.gov/pubmed/9512532
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