Design and validation of consensus-degenerate hybrid oligonucleotide primers for broad and sensitive detection of corona- and toroviruses

The ssRNA+ family Coronaviridae includes two subfamilies prototyped by coronaviruses and toroviruses that cause respiratory and enteric infections. To facilitate the identification of new distantly related members of the family Coronaviridae, we have developed a molecular assay with broad specificit...

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Veröffentlicht in:J Virol Methods
Hauptverfasser: Zlateva, Kalina T., Crusio, Kelly M., Leontovich, Andrey M., Lauber, Chris, Claas, Eric, Kravchenko, Alexander A., Spaan, Willy J.M., Gorbalenya, Alexander E.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Elsevier B.V. 2011
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112876/
https://ncbi.nlm.nih.gov/pubmed/21864579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2011.08.005
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