Design and validation of consensus-degenerate hybrid oligonucleotide primers for broad and sensitive detection of corona- and toroviruses

The ssRNA+ family Coronaviridae includes two subfamilies prototyped by coronaviruses and toroviruses that cause respiratory and enteric infections. To facilitate the identification of new distantly related members of the family Coronaviridae, we have developed a molecular assay with broad specificit...

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Udgivet i:J Virol Methods
Main Authors: Zlateva, Kalina T., Crusio, Kelly M., Leontovich, Andrey M., Lauber, Chris, Claas, Eric, Kravchenko, Alexander A., Spaan, Willy J.M., Gorbalenya, Alexander E.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier B.V. 2011
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7112876/
https://ncbi.nlm.nih.gov/pubmed/21864579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2011.08.005
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