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Site-directed point mutations in embryonic stem cells: a gene-targeting tag-and-exchange strategy.

Sequential gene targeting was used to introduce point mutations into one alpha 2 isoform Na,K-ATPase homolog in mouse embryonic stem (ES) cells. In the first round of targeted replacement, the gene was tagged with selectable markers by insertion of a Neor/HSV-tk gene cassette, and this event was sel...

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Gorde:
Xehetasun bibliografikoak
Argitaratua izan da:Mol Cell Biol
Egile Nagusiak: Askew, G R, Doetschman, T, Lingrel, J B
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Taylor & Francis 1993
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC359961/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8391633/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.7.4115-4124.1993
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