Site-directed point mutations in embryonic stem cells: a gene-targeting tag-and-exchange strategy.
Sequential gene targeting was used to introduce point mutations into one alpha 2 isoform Na,K-ATPase homolog in mouse embryonic stem (ES) cells. In the first round of targeted replacement, the gene was tagged with selectable markers by insertion of a Neor/HSV-tk gene cassette, and this event was sel...
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| Publicado no: | Mol Cell Biol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Taylor & Francis
1993
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC359961/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8391633/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.7.4115-4124.1993 |
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