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The illusion of specific capture: surface and solution studies of suboptimal oligonucleotide hybridization

BACKGROUND: Hybridization based assays and capture systems depend on the specificity of hybridization between a probe and its intended target. A common guideline in the construction of DNA microarrays, for instance, is that avoiding complementary stretches of more than 15 nucleic acids in a 50 or 60...

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Bibliografische gegevens
Hoofdauteurs: Garhyan, Jaishree, Gharaibeh, Raad Z, McGee, Stephen, Gibas, Cynthia J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: BioMed Central 2013
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3599332/
https://ncbi.nlm.nih.gov/pubmed/23445545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-6-72
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