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Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody
A serum-free medium (CHO-SFM) together with a fed-batch process was developed for the cultivation of a recombinant GS-CHO cell line producing TNFR-Fc. According to the metabolic characteristics of GS-CHO cell, a basal medium was prepared by supplementing DMEM:F12:RPMI1640 (2:1:1) with amino acids, i...
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| 主要な著者: | , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Springer Netherlands
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3597181/ https://ncbi.nlm.nih.gov/pubmed/22907508 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-012-9488-4 |
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