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Rational development of a serum-free medium and fed-batch process for a GS-CHO cell line expressing recombinant antibody
A serum-free medium (CHO-SFM) together with a fed-batch process was developed for the cultivation of a recombinant GS-CHO cell line producing TNFR-Fc. According to the metabolic characteristics of GS-CHO cell, a basal medium was prepared by supplementing DMEM:F12:RPMI1640 (2:1:1) with amino acids, i...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer Netherlands
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3597181/ https://ncbi.nlm.nih.gov/pubmed/22907508 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-012-9488-4 |
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