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Sequence verification of synthetic DNA by assembly of sequencing reads

Gene synthesis attempts to assemble user-defined DNA sequences with base-level precision. Verifying the sequences of construction intermediates and the final product of a gene synthesis project is a critical part of the workflow, yet one that has received the least attention. Sequence validation is...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Wilson, Mandy L., Cai, Yizhi, Hanlon, Regina, Taylor, Samantha, Chevreux, Bastien, Setubal, João C., Tyler, Brett M., Peccoud, Jean
Format: Artigo
Sprache:Inglês
Veröffentlicht: Oxford University Press 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592409/
https://ncbi.nlm.nih.gov/pubmed/23042248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks908
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