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MiniUIB, a Novel Minitransposon-Based System for Stable Insertion of Foreign DNA into the Genomes of Gram-Negative and Gram-Positive Bacteria

Transposition of the insertion sequence (IS) ISPpu12 is actively induced after conjugative interaction. The transposase of this IS can act in trans on structures flanked by inverted repeats similar to those of the transposon. Based on that fact, an ISPpu12-based minitransposon, miniUIB, has been con...

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Autori principali: Christie-Oleza, Joseph Alexander, Brunet-Galmés, Isabel, Lalucat, Jorge, Nogales, Balbina, Bosch, Rafael
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3591972/
https://ncbi.nlm.nih.gov/pubmed/23275505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03214-12
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