MiniUIB, a Novel Minitransposon-Based System for Stable Insertion of Foreign DNA into the Genomes of Gram-Negative and Gram-Positive Bacteria

Transposition of the insertion sequence (IS) ISPpu12 is actively induced after conjugative interaction. The transposase of this IS can act in trans on structures flanked by inverted repeats similar to those of the transposon. Based on that fact, an ISPpu12-based minitransposon, miniUIB, has been con...

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Bibliographic Details
Main Authors: Christie-Oleza, Joseph Alexander, Brunet-Galmés, Isabel, Lalucat, Jorge, Nogales, Balbina, Bosch, Rafael
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2013
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Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3591972/
https://ncbi.nlm.nih.gov/pubmed/23275505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03214-12
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