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Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris

The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutation...

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Main Authors: Hu, Hong, Gao, Jie, He, Jun, Yu, Bing, Zheng, Ping, Huang, Zhiqing, Mao, Xiangbing, Yu, Jie, Han, Guoquan, Chen, Daiwen
Formáid: Artigo
Teanga:Inglês
Foilsithe: Public Library of Science 2013
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3589435/
https://ncbi.nlm.nih.gov/pubmed/23472192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0058393
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