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Codon Optimization Significantly Improves the Expression Level of a Keratinase Gene in Pichia pastoris
The main keratinase (kerA) gene from the Bacillus licheniformis S90 was optimized by two codon optimization strategies and expressed in Pichia pastoris in order to improve the enzyme production compared to the preparations with the native kerA gene. The results showed that the corresponding mutation...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3589435/ https://ncbi.nlm.nih.gov/pubmed/23472192 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0058393 |
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