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Codon optimisation improves the expression of Trichoderma viride sp. endochitinase in Pichia pastoris

The mature cDNA of endochitinase from Trichoderma viride sp. was optimised based on the codon bias of Pichia pastoris GS115 and synthesised by successive PCR; the sequence was then transformed into P. pastoris GS115 via electroporation. The transformant with the fastest growth rate on YPD plates con...

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Detaylı Bibliyografya
Asıl Yazarlar: Yu, Ping, Yan, Yuan, Gu, Qing, Wang, Xiangyang
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group 2013
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3807108/
https://ncbi.nlm.nih.gov/pubmed/24154717
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03043
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