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Purification of proteins fused to glutathione S-tranferase
This chapter describes the use of glutathione S-transferase (GST) gene fusion proteins as a method for inducible, high-level protein expression and purification from bacterial cell lysates. The protein is expressed in a pGEX vector, with the GST moiety located at the N-terminus followed by the targe...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2011
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3584333/ https://ncbi.nlm.nih.gov/pubmed/20978970 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-913-0_14 |
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