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Nucleic acid amplification in vitro: detection of sequences with low copy numbers and application to diagnosis of human immunodeficiency virus type 1 infection.

The enzymatic amplification of specific nucleic acid sequences in vitro has revolutionized the use of nucleic acid hybridization assays for viral detection. With this method, the copy number of a pathogen-specific sequence is increased several orders of magnitude before detection is attempted. The s...

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Detalhes bibliográficos
Publicado no:Clin Microbiol Rev
Main Authors: Guatelli, J C, Gingeras, T R, Richman, D D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC358112/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2650862/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cmr.2.2.217
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