Nucleic acid amplification in vitro: detection of sequences with low copy numbers and application to diagnosis of human immunodeficiency virus type 1 infection.

The enzymatic amplification of specific nucleic acid sequences in vitro has revolutionized the use of nucleic acid hybridization assays for viral detection. With this method, the copy number of a pathogen-specific sequence is increased several orders of magnitude before detection is attempted. The s...

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Bibliographic Details
Published in:Clin Microbiol Rev
Main Authors: Guatelli, J C, Gingeras, T R, Richman, D D
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC358112/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2650862/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/cmr.2.2.217
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