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Artificial Environments for the Co-Translational Stabilization of Cell-Free Expressed Proteins

An approach for designing individual expression environments that reduce or prevent protein aggregation and precipitation is described. Inefficient folding of difficult proteins in unfavorable translation environments can cause significant losses of overexpressed proteins as precipitates or inclusio...

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Detalhes bibliográficos
Main Authors: Kai, Lei, Dötsch, Volker, Kaldenhoff, Ralf, Bernhard, Frank
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3579822/
https://ncbi.nlm.nih.gov/pubmed/23451062
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0056637
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