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Combining In vitro Folding with Cell-Free Protein Synthesis for Membrane Protein Expression

Cell free protein synthesis (CFPS) has emerged as a promising methodology for protein expression. While polypeptide production is very reliable and efficient using CFPS, the correct co-translational folding of membrane proteins during CFPS is still a challenge. In this contribution, we describe a tw...

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Detaylı Bibliyografya
Yayımlandı:Biochemistry
Asıl Yazarlar: Focke, Paul J., Hein, Christopher, Hoffmann, Beate, Matulef, Kimberly, Bernhard, Frank, Dötsch, Volker, Valiyaveetil, Francis I.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2016
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC5021318/
https://ncbi.nlm.nih.gov/pubmed/27384110
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.6b00488
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