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Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity
Specificity within the pathways of ubiquitin conjugation are defined by protein-binding affinities among the components. Enzyme kinetics provides a facile high-resolution experimental approach for quantitating such protein-binding affinities and yields additional mechanistic insights into the transi...
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| Main Authors: | , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2012
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3579653/ https://ncbi.nlm.nih.gov/pubmed/22350887 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-474-2_14 |
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