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Development of immunoblotting techniques for DNA radical detection

Radical damage to DNA has been implicated in cell death, cellular dysfunction and cancer. A recently developed method for detecting DNA radicals uses the nitrone spin trap DMPO (5,5-dimethyl-1-pyrroline N-oxide) to trap radicals. The trapped radicals then decay into stable nitrone adducts detectable...

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Main Authors: Summers, Fiona A., Mason, Ronald P., Ehrenshaft, Marilyn
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3577963/
https://ncbi.nlm.nih.gov/pubmed/23142572
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2012.10.550
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