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Linear-After-The-Exponential (LATE)–PCR: An advanced method of asymmetric PCR and its uses in quantitative real-time analysis

Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)–PCR describes a new paradigm for primer design that renders assays as eff...

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Autors principals: Sanchez, J. Aquiles, Pierce, Kenneth E., Rice, John E., Wangh, Lawrence J.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC357030/
https://ncbi.nlm.nih.gov/pubmed/14769930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0305476101
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