Two-temperature LATE-PCR endpoint genotyping

BACKGROUND: In conventional PCR, total amplicon yield becomes independent of starting template number as amplification reaches plateau and varies significantly among replicate reactions. This paper describes a strategy for reconfiguring PCR so that the signal intensity of a single fluorescent detect...

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Detalhes bibliográficos
Main Authors: Sanchez, J Aquiles, Abramowitz, Jessica D, Salk, Jesse J, Reis, Arthur H, Rice, John E, Pierce, Kenneth E, Wangh, Lawrence J
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2006
Assuntos:
Methodology Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698914/
https://ncbi.nlm.nih.gov/pubmed/17144924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-6-44
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