Design and Construction of a Single-Tube, LATE-PCR, Multiplex Endpoint Assay with Lights-On/Lights-Off Probes for the Detection of Pathogens Associated with Sepsis

Aims. The goal of this study was to construct a single tube molecular diagnostic multiplex assay for the detection of microbial pathogens commonly associated with septicemia, using LATE-PCR and Lights-On/Lights-Off probe technology. Methods and Results. The assay described here identified pathogens...

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Main Authors: Carver-Brown, Rachel K., Reis, Arthur H., Rice, Lisa M., Czajka, John W., Wangh, Lawrence J.
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2012
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3541598/
https://ncbi.nlm.nih.gov/pubmed/23326668
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2012/424808
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