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A Combined System for Engineering Glycosylation Efficiency and Glycan Structure in Saccharomyces cerevisiae

We describe a novel synthetic N-glycosylation pathway to produce recombinant proteins carrying human-like N-glycans in Saccharomyces cerevisiae, at the same time addressing glycoform and glycosylation efficiency. The Δalg3 Δalg11 double mutant strain, in which the N-glycans are not matured to their...

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Sonraí Bibleagrafaíochta
Main Authors: Parsaie Nasab, Farnoush, Aebi, Markus, Bernhard, Gesche, Frey, Alexander Daniel
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2013
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3568548/
https://ncbi.nlm.nih.gov/pubmed/23204425
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02817-12
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