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A Combined System for Engineering Glycosylation Efficiency and Glycan Structure in Saccharomyces cerevisiae
We describe a novel synthetic N-glycosylation pathway to produce recombinant proteins carrying human-like N-glycans in Saccharomyces cerevisiae, at the same time addressing glycoform and glycosylation efficiency. The Δalg3 Δalg11 double mutant strain, in which the N-glycans are not matured to their...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
American Society for Microbiology
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3568548/ https://ncbi.nlm.nih.gov/pubmed/23204425 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02817-12 |
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