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Quantitative Measurement of Protein Relocalization in Live Cells
Microscope cytometry provides a powerful means to study signaling in live cells. Here we present a quantitative method to measure protein relocalization over time, which reports the absolute fraction of a tagged protein in each compartment. Using this method, we studied an essential step in the earl...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3566451/ https://ncbi.nlm.nih.gov/pubmed/23442923 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.12.030 |
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