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Measuring Affinities of Fission Yeast Spindle Pole Body Proteins in Live Cells across the Cell Cycle

Characterizing protein-protein interactions is essential for understanding molecular mechanisms, although reproducing cellular conditions in vitro is challenging and some proteins are difficult to purify. We developed a method to measure binding to cellular structures using fission yeast cells as re...

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Main Authors: McCormick, Chad D., Akamatsu, Matthew S., Ti, Shih-Chieh, Pollard, Thomas D.
Formáid: Artigo
Teanga:Inglês
Foilsithe: The Biophysical Society 2013
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3827966/
https://ncbi.nlm.nih.gov/pubmed/24047983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.08.017
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