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Measuring Affinities of Fission Yeast Spindle Pole Body Proteins in Live Cells across the Cell Cycle
Characterizing protein-protein interactions is essential for understanding molecular mechanisms, although reproducing cellular conditions in vitro is challenging and some proteins are difficult to purify. We developed a method to measure binding to cellular structures using fission yeast cells as re...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
The Biophysical Society
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3827966/ https://ncbi.nlm.nih.gov/pubmed/24047983 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2013.08.017 |
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