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Automatic and Quantitative Measurement of Protein-Protein Colocalization in Live Cells

We introduce a novel statistical approach that quantifies, for the first time, the amount of colocalization of two fluorescent-labeled proteins in an image automatically, removing the bias of visual interpretation. This is done by estimating simultaneously the maximum threshold of intensity for each...

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Detalhes bibliográficos
Main Authors: Costes, Sylvain V., Daelemans, Dirk, Cho, Edward H., Dobbin, Zachary, Pavlakis, George, Lockett, Stephen
Formato: Artigo
Idioma:Inglês
Publicado em: Biophysical Society 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304300/
https://ncbi.nlm.nih.gov/pubmed/15189895
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.103.038422
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