An M13 bacteriophage display framework that allows sortase-mediated modification of surface-accessible phage proteins

We exploit bacterial sortases to attach a variety of moieties to the capsid proteins of M13 bacteriophage. We show that pIII, pIX, and pVIII can be functionalized with entities ranging from small molecules (e.g., fluorophores, biotin) to correctly folded proteins (e.g., GFP, antibodies, streptavidin...

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Hauptverfasser: Hess, Gaelen T., Cragnolini, Juan J., Popp, Maximilian W., Allen, Mark A., Dougan, Stephanie K., Spooner, Eric, Ploegh, Hidde L., Belcher, Angela M., Guimaraes, Carla P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3564602/
https://ncbi.nlm.nih.gov/pubmed/22759232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc300130z
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