An M13 bacteriophage display framework that allows sortase-mediated modification of surface-accessible phage proteins

We exploit bacterial sortases to attach a variety of moieties to the capsid proteins of M13 bacteriophage. We show that pIII, pIX, and pVIII can be functionalized with entities ranging from small molecules (e.g., fluorophores, biotin) to correctly folded proteins (e.g., GFP, antibodies, streptavidin...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Hess, Gaelen T., Cragnolini, Juan J., Popp, Maximilian W., Allen, Mark A., Dougan, Stephanie K., Spooner, Eric, Ploegh, Hidde L., Belcher, Angela M., Guimaraes, Carla P.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2012
Pynciau:
Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3564602/
https://ncbi.nlm.nih.gov/pubmed/22759232
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bc300130z
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg