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Protein Ligation in Living Cells Using Sortase
Sortagging is a versatile method for site-specific modification of proteins as applied to a variety of in vitro reactions. Here, we explore possibilities of adapting the sortase method for use in living cells. For intracellular sortagging, we employ the Ca(2+)-independent sortase A transpeptidase (S...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Blackwell Publishing Ltd
2012
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3490390/ https://ncbi.nlm.nih.gov/pubmed/22348280 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1600-0854.2012.01345.x |
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