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Protein Ligation in Living Cells Using Sortase

Sortagging is a versatile method for site-specific modification of proteins as applied to a variety of in vitro reactions. Here, we explore possibilities of adapting the sortase method for use in living cells. For intracellular sortagging, we employ the Ca(2+)-independent sortase A transpeptidase (S...

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Bibliografische gegevens
Hoofdauteurs: Strijbis, Karin, Spooner, Eric, Ploegh, Hidde L
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Blackwell Publishing Ltd 2012
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3490390/
https://ncbi.nlm.nih.gov/pubmed/22348280
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1600-0854.2012.01345.x
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