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Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Transposon mutagenesis and single-gene deletion are two methods applied in genome-wide gene knockout in bacteria (1,2). Although transposon mutagenesis is less time consuming, less costly, and does not require completed genome information, there are two weaknesses in this method: (1) the possibility...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2012
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3563760/ https://ncbi.nlm.nih.gov/pubmed/23207952 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/4356 |
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