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Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation
Essential genes in pathogens are important for the development of antibacterial drugs. In this report, we described a protocol to identify essential genes in the Streptococcus sanguinis SK36 strain using genome-wide deletion mutation. A fusion PCR-based method is used to construct gene deletion frag...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4819415/ https://ncbi.nlm.nih.gov/pubmed/25636610 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2398-4_2 |
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