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Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method

Gene overexpression beyond a permissible limit causes defects in cellular functions. However, the permissible limits of most genes are unclear. Previously, we developed a genetic method designated genetic tug-of-war (gTOW) to measure the copy number limit of overexpression of a target gene. In the c...

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Detalhes bibliográficos
Main Authors: Makanae, Koji, Kintaka, Reiko, Makino, Takashi, Kitano, Hiroaki, Moriya, Hisao
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3561871/
https://ncbi.nlm.nih.gov/pubmed/23275495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.146662.112
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