Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW

BACKGROUND: Identifying permissible limits of intracellular parameters such as protein expression provides important information for examining robustness. In this study, we used the TEV protease-mediated induction of protein instability (TIPI) in combination with the genetic Tug-of-War (gTOW) to dev...

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Hauptverfasser: Sasabe, Masataka, Shintani, Sayumi, Kintaka, Reiko, Kaizu, Kazunari, makanae, Koji, Moriya, Hisao
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2014
Schlagworte:
Methodology Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3892067/
https://ncbi.nlm.nih.gov/pubmed/24393197
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1752-0509-8-2
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