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Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW
BACKGROUND: Identifying permissible limits of intracellular parameters such as protein expression provides important information for examining robustness. In this study, we used the TEV protease-mediated induction of protein instability (TIPI) in combination with the genetic Tug-of-War (gTOW) to dev...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2014
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3892067/ https://ncbi.nlm.nih.gov/pubmed/24393197 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1752-0509-8-2 |
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