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Structure and dynamics of a primordial catalytic fold generated by in vitro evolution
Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a non-catalytic protein scaffold, we recently generated a novel RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopte...
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| 主要な著者: | , , , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2012
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3552148/ https://ncbi.nlm.nih.gov/pubmed/23222886 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.1138 |
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