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Rapid and specific purification of Argonaute-small RNA complexes from crude cell lysates
Small interfering RNAs (siRNAs) direct Argonaute proteins, the core components of the RNA-induced silencing complex (RISC), to cleave complementary target RNAs. Here, we describe a method to purify active RISC containing a single, unique small RNA guide sequence. We begin by capturing RISC using a c...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3543083/ https://ncbi.nlm.nih.gov/pubmed/23249751 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.036921.112 |
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