Large Multiple Transmembrane Domain Fragments of a G Protein-Coupled Receptor: Biosynthesis, Purification, and Biophysical Studies

To conduct biophyiscal analyses on large domains of GPCRs, multi-milligram quantities of highly homogeneous proteins are necessary. This communication discusses the biosynthesis of 4 transmembrane and 5 transmembrane-containing fragments of Ste2p, a GPCR recognizing the Saccharomyces cerevisiae trid...

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Bibliographic Details
Main Authors: Potetinova, Zhanna, Tantry, Subramanyam, Cohen, Leah S., Caroccia, Katrina E., Arshava, Boris, Becker, Jeffrey M., Naider, Fred
Format: Artigo
Language:Inglês
Published: 2012
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3542537/
https://ncbi.nlm.nih.gov/pubmed/23203693
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.22122
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