Guided Reconstitution of Membrane Protein Fragments

Structural analysis by NMR of G protein-coupled receptors (GPCRs) has proven to be extremely challenging. To reduce the number of peaks in the NMR spectra by segmentally-labeling a GPCR, we have developed a Guided Reconstitution method that includes the use of charged residues and Cys activation to...

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Detalhes bibliográficos
Publicado no:Biopolymers
Main Authors: Cohen, Leah S., Arshava, Boris, Kauffman, Sarah, Mathew, Elizabeth, Fracchiolla, Katrina E., Ding, Fa-Xiang, Dumont, Mark, Becker, Jeffrey M., Naider, Fred
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4246424/
https://ncbi.nlm.nih.gov/pubmed/23897574
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.22349
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