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A modular assembly cloning technique (aided by the BIOF software tool) for seamless and error-free assembly of long DNA fragments
BACKGROUND: Molecular cloning of DNA fragments >5 kbp is still a complex task. When no genomic DNA library is available for the species of interest, and direct PCR amplification of the desired DNA fragment is unsuccessful or results in an incorrect sequence, molecular cloning of a PCR-amplified r...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3539906/ https://ncbi.nlm.nih.gov/pubmed/22709633 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-303 |
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