Mass spectrometry analysis of human P2X1 receptors; insight into phosphorylation, modelling and conformational changes

Recombinant FlagHis(6) tagged Human P2X1 receptors expressed in HEK293 cells were purified, digested with trypsin and analysed by mass spectroscopy (96% coverage following de-glycosylation and reduction). The receptor was basally phosphorylated at residues S387, S388 and T389 in the carboxyl terminu...

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Detalhes bibliográficos
Main Authors: Roberts, Jonathan A, Bottrill, Andrew R, Mistry, Sharad, Evans, Richard J
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Publishing Ltd 2012
Assuntos:
Original Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3532615/
https://ncbi.nlm.nih.gov/pubmed/22971236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/jnc.12012
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