Apolar Distal Pocket Mutants of Yeast Cytochrome c Peroxidase: Hydrogen Peroxide Reactivity and Cyanide Binding of the TriAla, TriVal, and TriLeu Variants

Three yeast cytochrome c peroxidase (CcP) variants with apolar distal heme pockets have been constructed. The CcP variants have Arg48, Trp51, and His52 mutated to either all alanines, CcP(triAla), all valines, CcP(triVal), or all leucines, CcP(triLeu). The triple mutants have detectable enzymatic ac...

Full description

Saved in:
Bibliographic Details
Main Authors: Bidwai, Anil K., Meyen, Cassandra, Kilheeney, Heather, Wroblewski, Damian, Vitello, Lidia B., Erman, James E.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3530626/
https://ncbi.nlm.nih.gov/pubmed/23022490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2012.09.005
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items