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Apolar Distal Pocket Mutants of Yeast Cytochrome c Peroxidase: Hydrogen Peroxide Reactivity and Cyanide Binding of the TriAla, TriVal, and TriLeu Variants

Three yeast cytochrome c peroxidase (CcP) variants with apolar distal heme pockets have been constructed. The CcP variants have Arg48, Trp51, and His52 mutated to either all alanines, CcP(triAla), all valines, CcP(triVal), or all leucines, CcP(triLeu). The triple mutants have detectable enzymatic ac...

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Detalhes bibliográficos
Main Authors: Bidwai, Anil K., Meyen, Cassandra, Kilheeney, Heather, Wroblewski, Damian, Vitello, Lidia B., Erman, James E.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3530626/
https://ncbi.nlm.nih.gov/pubmed/23022490
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbapap.2012.09.005
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