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Atomic Scissors: A New Method of Tracking the 5-Bromo-2′-Deoxyuridine-Labeled DNA In Situ

A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I) in the presence of oxygen, which leads to the abstraction of hydrogen atom from deoxyribose culminating in the elimination of the nucleo...

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Detalhes bibliográficos
Main Authors: Ligasová, Anna, Strunin, Dmytro, Liboska, Radek, Rosenberg, Ivan, Koberna, Karel
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3530445/
https://ncbi.nlm.nih.gov/pubmed/23300711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0052584
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